Pepper Seed Dna Extraction - 2422 Words

Pepper Seed DNA Extraction Biochem lab: CHE 452L marisol gomez Pepper Seed DNA Extraction Biochem lab: CHE 452L marisol gomez 2015 2015 INTRODUCTION The jalapeno is a member of the capsicum family, along with many other peppers. The usual methods for characterization of different pepper species are based on their morphological and physiological traits, however this many not always be enough. For peppers, their traits are influenced by things like their genotype or their specific environment. Genomic markers can allow for a more direct comparison of closely related individuals (Ansari and Khan, 2012). In our case we focus on DNA extraction. The two basic parts of a DNA extraction procedure include the breaking of the cell walls†¦show more content†¦Reliable DNA analysis is also important in the management of wild life. In the case of Wedrowicz, the koala population is being threatened and the need for data on their genetic diversity is of extreme importance. However obtaining the DNA from the koala is not a simple task, this is due to cost, stress, and ethics of capturing a koala. Sometimes the only way to obtain a sample of DNA is by the collection of scats. This means that once the DNA sample is obtained it must be rushed to the lab for extraction while the sample is still fresh. Obtaining this DNA can allow for analysis of population, breeding, and habitat of not only koalas, but of many other mammals (Wedrowicz, Faye, 2013). MATERIALS AND METHODS Plant Materials Seeds from different capsicum plants were purchased locally. The pepper seeds collected were ‘Japanese chili’, ‘California Bell’, ‘Serrano’, ‘Habanero’, and ‘Jalapeno’. Chemicals and Reagents The chemical and reagents used for the extraction and quantitation of DNA were: Plant DNAzol (0.3ml/0.1g), 100% ethanol (100%: 0.225 ml/0.1 g, 75%: 0.3 ml/0.1 g), Chloroform (0.3 ml/0.1 g), Plant DNAzol-ethanol solution: Plant DNAzol, 100% ethanol (1:0.75 v/v), TE buffer (10 mM Tris, 1 mM EDTA pH 8.0), 1.2% agarose gel (Agarose, 1X TAE buffer), 6X loading buffer (glycerol, Tris/EDTA pH 8.0, ethidium bromide), .25X TAE buffer, Restriction enzymes and Restriction endonuclease buffers. All the chemicals used were quality grade. The restrictionShow MoreRelatedGenetic Engineering And Its Effect On Human Health1996 Words   |  8 PagesIntroduction Genetically modified crops also known as GMCs, GM crops or biotech crops, are plants, which DNA has been modified using genetic engineering techniques. In most cases the goal is to introduce a new trait to the plant, which does not occur naturally in the species, for examples in crops and food. Since 1960, there are many introducing genetic engineering in crops production as it can bring a lot of benefits e.g. increasing food production, it is also environmental friendly and it givesRead MoreThe Process Of Genetic Engineering2336 Words   |  10 Pageslist-------------------------------------------------------------------------------------------------------------- Introduction Genetically modified crops also known, as GMCs, GM crops, or biotech crops, genetic modified crops are plants used in agriculture, the DNA of which has been modified using genetic engineering techniques. In most events the goal is to introduce a new trait to the plant, which does not occur naturally in the species, for examples in crops and food. Since from 1960, there are many countriesRead MorePharmacological And Ethnobotanical Review Of Aristolochia Bracteolata L4386 Words   |  18 PagesKida-mari, Ethnomedicines. Introduction Plant Perennial scandent shrubs, leaves raniform or ovate, usually obtuse, cordate at the base with a wide shallow sinous. Flowers solitary, dark purple. Capsules oblong, ellipsoid, and 12- ribbed, glabrous. Seeds deltoid, with slightly cordate base. Slender, decumbent, glabrous, perennial herbs, woody at base. Leaves broadly ovate or reniform, 3-6 x 4.5-8 cm, glaucous below. Flowers dark purple, axillary, solitary. Capsules oblong-ellipsoid, 1.5-2.2 x 1-1.5Read MoreSexually Transmitted Diseases35655 Words   |  143 Pages 114-128 Unit 6 Living things in the Environment Plant and animal cells 129 Eukaryotes vs prokaryotes 130 Structures and functions 133-137 Microbes 148-150 Onion and cheek cells 150-151 Ecological study 154 Seed structure and germination 155-157 Reproduction in plants 158-165 Sexually transmitted diseases 166-176 Birth Control and contraceptives 177-185 Effects on population growth 186-187 Drug and drug abuse 188-191 Man’s impact