Pepper Seed Dna Extraction - 2422 Words

Pepper Seed DNA Extraction Biochem lab: CHE 452L marisol gomez Pepper Seed DNA Extraction Biochem lab: CHE 452L marisol gomez 2015 2015 INTRODUCTION The jalapeno is a member of the capsicum family, along with many other peppers. The usual methods for characterization of different pepper species are based on their morphological and physiological traits, however this many not always be enough. For peppers, their traits are influenced by things like their genotype or their specific environment. Genomic markers can allow for a more direct comparison of closely related individuals (Ansari and Khan, 2012). In our case we focus on DNA extraction. The two basic parts of a DNA extraction procedure include the breaking of the cell walls†¦show more content†¦Reliable DNA analysis is also important in the management of wild life. In the case of Wedrowicz, the koala population is being threatened and the need for data on their genetic diversity is of extreme importance. However obtaining the DNA from the koala is not a simple task, this is due to cost, stress, and ethics of capturing a koala. Sometimes the only way to obtain a sample of DNA is by the collection of scats. This means that once the DNA sample is obtained it must be rushed to the lab for extraction while the sample is still fresh. Obtaining this DNA can allow for analysis of population, breeding, and habitat of not only koalas, but of many other mammals (Wedrowicz, Faye, 2013). MATERIALS AND METHODS Plant Materials Seeds from different capsicum plants were purchased locally. The pepper seeds collected were ‘Japanese chili’, ‘California Bell’, ‘Serrano’, ‘Habanero’, and ‘Jalapeno’. Chemicals and Reagents The chemical and reagents used for the extraction and quantitation of DNA were: Plant DNAzol (0.3ml/0.1g), 100% ethanol (100%: 0.225 ml/0.1 g, 75%: 0.3 ml/0.1 g), Chloroform (0.3 ml/0.1 g), Plant DNAzol-ethanol solution: Plant DNAzol, 100% ethanol (1:0.75 v/v), TE buffer (10 mM Tris, 1 mM EDTA pH 8.0), 1.2% agarose gel (Agarose, 1X TAE buffer), 6X loading buffer (glycerol, Tris/EDTA pH 8.0, ethidium bromide), .25X TAE buffer, Restriction enzymes and Restriction endonuclease buffers. All the chemicals used were quality grade. The restrictionShow MoreRelatedGenetic Engineering And Its Effect On Human Health1996 Words   |  8 PagesIntroduction Genetically modified crops also known as GMCs, GM crops or biotech crops, are plants, which DNA has been modified using genetic engineering techniques. In most cases the goal is to introduce a new trait to the plant, which does not occur naturally in the species, for examples in crops and food. Since 1960, there are many introducing genetic engineering in crops production as it can bring a lot of benefits e.g. increasing food production, it is also environmental friendly and it givesRead MoreThe Process Of Genetic Engineering2336 Words   |  10 Pageslist-------------------------------------------------------------------------------------------------------------- Introduction Genetically modified crops also known, as GMCs, GM crops, or biotech crops, genetic modified crops are plants used in agriculture, the DNA of which has been modified using genetic engineering techniques. In most events the goal is to introduce a new trait to the plant, which does not occur naturally in the species, for examples in crops and food. Since from 1960, there are many countriesRead MorePharmacological And Ethnobotanical Review Of Aristolochia Bracteolata L4386 Words   |  18 PagesKida-mari, Ethnomedicines. Introduction Plant Perennial scandent shrubs, leaves raniform or ovate, usually obtuse, cordate at the base with a wide shallow sinous. Flowers solitary, dark purple. Capsules oblong, ellipsoid, and 12- ribbed, glabrous. Seeds deltoid, with slightly cordate base. Slender, decumbent, glabrous, perennial herbs, woody at base. Leaves broadly ovate or reniform, 3-6 x 4.5-8 cm, glaucous below. Flowers dark purple, axillary, solitary. Capsules oblong-ellipsoid, 1.5-2.2 x 1-1.5Read MoreSexually Transmitted Diseases35655 Words   |  143 Pages 114-128 Unit 6 Living things in the Environment Plant and animal cells 129 Eukaryotes vs prokaryotes 130 Structures and functions 133-137 Microbes 148-150 Onion and cheek cells 150-151 Ecological study 154 Seed structure and germination 155-157 Reproduction in plants 158-165 Sexually transmitted diseases 166-176 Birth Control and contraceptives 177-185 Effects on population growth 186-187 Drug and drug abuse 188-191 Man’s impact

Role Of The President And Congress During The Economy Essay

Introduction The economic conditions prevailing in a country impact heavily on local businesses and organization. Depending on how well prepares an organization is prepared to handle the economic changes that occur, the organization can either thrive in business or collapse. There is more to this picture. An individual organization cannot shield itself enough against harsh economic tides. Thus, the government has to create checks and balances to bring about a stable and sustainable economic environment so as to spur a steady economic growth for local businesses and firms to thrive. Role of the President and Congress in the Economy The President works hand in hand with congress to make policies that either stimulate or contract the economy.Such policies may be made to either increase or reduce government spending on such sectors as defense, in terms of military spending, health, education and other sectors. Primarily, the government must spend in order to develop infrastructure, provide basic services to the people as well as enforce laws and contracts on the land (Mitchell, 2005). Without such expenditure, there would be little, if any, economic progress. However, a balance must be maintained to ensure that this expenditure does not exceed what the nation can afford. The government must, therefore, â€Å"live within its means.† With increased spending, the government tends to increase taxes in order to meet the cost. This in reverse, threatens productivity among theShow MoreRelated Congress Essay1734 Words   |  7 Pagesfor Congress in the United State s. It serves many roles such as making laws, implementing national policy and watching over the other two branches of government. These are just a few of the duties of our U.S. Congress. Although they are essential to our government, there are potential problems. 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Forecast of Aud/Usd Free Essays

In short term, Australia is facing higher inflation rate (2. 9%) than the United States (2. 1%), this can lead to the domestic market price increase but not much fluctuation for currency exchange rate. We will write a custom essay sample on Forecast of Aud/Usd: or any similar topic only for you Order Now Moreover, Australia has higher GDP growth rate than United States (3. 5% vs 2. 4%) and lower unemployment rate than US, which are 5. 2% and 7. 9% in 2013. Therefore, the income will increase in Australia. Australia consumers would spend more on the US imports and the demand for US will increase. The supply of dollars will increase to buy more US products and the value of USD will increase relative to the AUD. In conclusion, we predict that the exchange rate of AUD/USD will decrease in the short term. In intermediate term, there will be almost a constant decline of inflation rate each year in Australia while that of United States remains fairly constant. The Inflation rate in United States is still lower than Australia. As a result, it is highly unlikely that the interest rate in Australia will increase by a large amount. On the other hand, the interest rate within the United States would be expected to remain quite low. In addition, according to the information from IMF, GDP growth of Australia will be stable at 3. 5% while that of United States will increase from 2. 4% to 3. 5%. Although there is a large reduction in unemployment rate in United States, it is still higher than Australia’s. Therefore, as long as the US Federal Reserve will not implement the monetary policy QE3 in the next few years, the currency of the United States will go up against Australia, but exchange rate of Australia will still be slightly higher than United States in the intermediate term. In the long term (greater than 5 years), the inflation rate of Australia will drop to 2. 4% in 2017 while that of United States will start to rise. On the other hand, GDP growth for both Australia and United States are similar at a stable pace of 4% and are hard to improve?In addition, the unemployment rate will continue to decline to about 4%. After 2017, inflation rates and GDP growth rate of both countries are expected to get closer and closer, achieving the stable coordination finally. At the same time, the Central Banks should not raise their interest rate to lower the inflation. Therefore, in conclusion, we predict that the exchange rate of AUD/USD will remain at a stable relationship with each other in the long term. Reference: IMF 2012, IMF Data Mapper, 2Executive summary This report focuses on forecasting the trend of exchange rate of AUD/USD and PHP/USD in short term, intermediate term and long term. The fundamental analysis is based on the changes in demand and supply of those three currencies. In terms of PHP/USD, for short term, the higher domestic interest rate in Philippine and the monetary policy (QE) will be implemented by United States will result in PHP/USD decreases. For intermediate term, as QE grows the GDP of United States, Philippines remain domestic policies and target, the supply of dollars increase faster than demand of pesos decrease, PHP/USD continue to decrease. For long term, those factors which affect the exchange rate will trend to reach an another equilibrium, PHP/USD increase. As for AUD/USD, due to the increased consumption of Australia on the US imports, the demand for US will increase. Consequently, AUD/USD will decrease in the short term. For intermediate term, Unit Stated will experience an obvious economic recovery while the major macroeconomic variables in Australia generally remain at a stable level, thus AUD/USD will continuous fall, but AUD will still appreciate slightly against USD. In the long term, the economy of both countries are expected to get a stable coordination, so it is likely that the USD will stop depreciating against the AUD and the exchange rate between the USD and he AUD achieve an new equilibrium. In recommendation, US dollar trend to depreciate against Philippines peso in short and intermediate term, purchasing pesos using US dollars now and exchange back to US dollar in the future which up to 5 years for gaining profit. US dollar trend to be appreciated against AU dollar in short and intermediate term, hence the top manager should hold a short position on the AUD and a long position on the USD, while for the long term the top manager just hold a short position on the USD. How to cite Forecast of Aud/Usd:, Papers

Forecast of Aud/Usd Free Essays

In short term, Australia is facing higher inflation rate (2. 9%) than the United States (2. 1%), this can lead to the domestic market price increase but not much fluctuation for currency exchange rate. We will write a custom essay sample on Forecast of Aud/Usd: or any similar topic only for you Order Now Moreover, Australia has higher GDP growth rate than United States (3. 5% vs 2. 4%) and lower unemployment rate than US, which are 5. 2% and 7. 9% in 2013. Therefore, the income will increase in Australia. Australia consumers would spend more on the US imports and the demand for US will increase. The supply of dollars will increase to buy more US products and the value of USD will increase relative to the AUD. In conclusion, we predict that the exchange rate of AUD/USD will decrease in the short term. In intermediate term, there will be almost a constant decline of inflation rate each year in Australia while that of United States remains fairly constant. The Inflation rate in United States is still lower than Australia. As a result, it is highly unlikely that the interest rate in Australia will increase by a large amount. On the other hand, the interest rate within the United States would be expected to remain quite low. In addition, according to the information from IMF, GDP growth of Australia will be stable at 3. 5% while that of United States will increase from 2. 4% to 3. 5%. Although there is a large reduction in unemployment rate in United States, it is still higher than Australia’s. Therefore, as long as the US Federal Reserve will not implement the monetary policy QE3 in the next few years, the currency of the United States will go up against Australia, but exchange rate of Australia will still be slightly higher than United States in the intermediate term. In the long term (greater than 5 years), the inflation rate of Australia will drop to 2. 4% in 2017 while that of United States will start to rise. On the other hand, GDP growth for both Australia and United States are similar at a stable pace of 4% and are hard to improve?In addition, the unemployment rate will continue to decline to about 4%. After 2017, inflation rates and GDP growth rate of both countries are expected to get closer and closer, achieving the stable coordination finally. At the same time, the Central Banks should not raise their interest rate to lower the inflation. Therefore, in conclusion, we predict that the exchange rate of AUD/USD will remain at a stable relationship with each other in the long term. Reference: IMF 2012, IMF Data Mapper, 2Executive summary This report focuses on forecasting the trend of exchange rate of AUD/USD and PHP/USD in short term, intermediate term and long term. The fundamental analysis is based on the changes in demand and supply of those three currencies. In terms of PHP/USD, for short term, the higher domestic interest rate in Philippine and the monetary policy (QE) will be implemented by United States will result in PHP/USD decreases. For intermediate term, as QE grows the GDP of United States, Philippines remain domestic policies and target, the supply of dollars increase faster than demand of pesos decrease, PHP/USD continue to decrease. For long term, those factors which affect the exchange rate will trend to reach an another equilibrium, PHP/USD increase. As for AUD/USD, due to the increased consumption of Australia on the US imports, the demand for US will increase. Consequently, AUD/USD will decrease in the short term. For intermediate term, Unit Stated will experience an obvious economic recovery while the major macroeconomic variables in Australia generally remain at a stable level, thus AUD/USD will continuous fall, but AUD will still appreciate slightly against USD. In the long term, the economy of both countries are expected to get a stable coordination, so it is likely that the USD will stop depreciating against the AUD and the exchange rate between the USD and he AUD achieve an new equilibrium. In recommendation, US dollar trend to depreciate against Philippines peso in short and intermediate term, purchasing pesos using US dollars now and exchange back to US dollar in the future which up to 5 years for gaining profit. US dollar trend to be appreciated against AU dollar in short and intermediate term, hence the top manager should hold a short position on the AUD and a long position on the USD, while for the long term the top manager just hold a short position on the USD. How to cite Forecast of Aud/Usd:, Papers